cd86 pe 65068  (Proteintech)

Journal: Advanced Science

Article Title: Synergistic Modulation of Microglial Polarization by Acteoside and Ferulic Acid via Dual Targeting of Nrf2 and RORγt to Alleviate Depression‐Associated Neuroinflammation

doi: 10.1002/advs.202503889

Figure Lengend Snippet: Cooperative effects of ACT and FA on microglial polarization in the LPS‐induced BV2 cells. The effects of FA A) and ACT B) on BV2 cells viability. Effects of FA or ACT single treatments on the levels of IL‐6, IL‐17A C), IL‐1β, and IL‐10 D). E) Neuroprotection of ACT synergistic FA on LPS‐induced BV2 cell morphology (100 µm). F) Statistical analysis of co‐treatment of ACT synergistic FA replicated the equivalent ratio of them in the BDD on the M1 and M2 polarization markers of microglia‐associated mRNAs expression. G) Expression level of the IL‐6, IL‐17A, IL‐1β, and IL‐10 in the supernatant of BV2 cells. H) Flow cytometry of CD86 and CD206 in the LPS‐induced BV2 cells. Quantitative analysis of Nrf2, TREM2, and DAP12 mRNAs I) and proteins J) expression level. GAPDH was set as the internal control. Results are presented as the mean ± SEM of three independent experiments. * p <0.05, ** p <0.01 and *** p <0.001, compared with the control group; # p <0.05, ## p <0.01 and ### p <0.001, compared with the LPS‐induced cell model group; & P <0.05, && P <0.01 and &&& P <0.001, compared with the ACT+FA treatment group.

Article Snippet: For microglial polarization phenotype analysis, BV‐2 cells were harvested 24 h after treatment, washed sequentially in ice‐cold flow buffer, and incubated with specific antibodies against CD206 (bsm‐30276A‐FITC; Bioss) and CD86 (bsm‐30162A‐PE; Bioss).

Techniques: Expressing, Flow Cytometry, Control

Journal: Advanced Science

Article Title: Synergistic Modulation of Microglial Polarization by Acteoside and Ferulic Acid via Dual Targeting of Nrf2 and RORγt to Alleviate Depression‐Associated Neuroinflammation

doi: 10.1002/advs.202503889

Figure Lengend Snippet: siRNA knockdown of Nrf2 reverses the phenotypic transformation induced by ACT and FA in BV2 cells. A) Schematic diagram of plasmid transfection efficiency with GFP fluorescence in different time points (1/8/16/24 h), and qRT‐PCR was used to detect the knockdown efficiency of siRNA targeted Nrf2. B) The expression levels of IL‐1β, IL‐6, IL‐17A, and IL‐10 in BV2 cells were tested by ELISA. C)The levels of polarization biomarkers of CD86 and CD206 were detected by flow cytograms of BV2 cells in each group. D) Relative mRNA expression levels of M1 and M2 markers, and Nrf2/TREM2/DAP12 in the different groups. Results are presented as the mean ± SEM of three independent experiments. * p <0.05, ** p <0.01, and *** p <0.001, compared with the Si‐NC group; # p <0.05, ## p <0.01, and ### p <0.001, compared with the LPS‐induced cell model treated by ACT and FA.

Article Snippet: For microglial polarization phenotype analysis, BV‐2 cells were harvested 24 h after treatment, washed sequentially in ice‐cold flow buffer, and incubated with specific antibodies against CD206 (bsm‐30276A‐FITC; Bioss) and CD86 (bsm‐30162A‐PE; Bioss).

Techniques: Knockdown, Transformation Assay, Plasmid Preparation, Transfection, Fluorescence, Quantitative RT-PCR, Expressing, Enzyme-linked Immunosorbent Assay